Specific Serum Agglutination of Erythrocytes Sensitized with Extracts of Tubercle Bacilli
نویسندگان
چکیده
A hemagglutination reaction has been described between sheep erythrocytes treated with a component of a polysaccharide fraction of mammalian tubercle bacilli and the sera of experimental animals or of tuberculous patients. Evidence has been presented for the specificity of this reaction. A modification of the test, involving an inhibition reaction, has been developed for the detection and quantitation of minute amounts of the material responsible for the hemagglutination reaction.
منابع مشابه
The erythrocytes sensitized with tuberculin-polysaccaride in the circulating blood: its diagnostic application for early stage of tuberculosis in children.
(Received for publication, December 24, 1957) In tuberculous cases of children, the erythrocytes sensitized with tuberculinpolysaccharide were detected by means of the agglutination reaction between the erythrocytes and the immune serum prepared in the following way. Sheep were treated by repeated injection of killed tubercle bacilli, until the serum ob tained from same was able to cause an agg...
متن کاملSpecific lytic reaction of bovine erythrocytes sensitized with tuberculin, and its diagnostic value upon tuberculosis.
Serological methods for the detection of antibody in the sera of tuberculous patients have been often tested, but the result has only ascertained that the complement fixation test has a supplementary dia gnostic value. In 1948, Middlebrook and Dubos1) proved a hemag glutination in tuberculous sera, using sheep erythrocytes sensitized with polysaccharide fraction of human tubercle bacilli (H37Rv...
متن کاملOccurrence of the erythrocytes sensitized with tuberculin-polysaccharide in the circulating blood of the rabbits with tuberculous infection.
(Received for publication, December 24, 1957) When 40 mg of living tubercle bacilli of Aoyama-B-strain, suspended in 4 cc of saline, were given intratracheally to rabbits, the erythrocytes sensitized with tuberculin-polysaccharide were demonstrated in the circulating blood on the 3rd, 4th and 5th days after the inoculation with the bacilli, but not found after the 6th day, when the antibody aga...
متن کاملA hemolytic reaction of erythrocytes sensitized with tuberculin and components extracted from leprosy nodules.
In recent years, Middlebrook and Dubos1) has published a hemag glutination reaction of erythrocytes sensitized with extracts of tubercle bacilli and it was retested by many investigators. Subsequently the test was made in leprosy by Levine2) with sheep red cells sensitized with tuberculin. It was revealed that the test was applicable for measuring a serological responce in leprosy patients and ...
متن کاملThe Specificity of the Second Stage of Bacterial Agglutination and Hemagglutination.
About 40 years ago Bordet demonstrated that agglutinins did not ordinarily agglutinate bacilli unless salt was present, and until recently it has been customary for all immunologists to regard the process of specific agglutination as involving two separate stages; first, a primary, specific, and rapid combination of antibody with antigen, and second, a slower stage-requiring the presence of an ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 88 شماره
صفحات -
تاریخ انتشار 1948